Brad Day

Principle Investigator


Brad Day Ph.D.

Plant, Soil and Microbial Sciences



1990-1994, The University of Tennessee. B.S., Microbiology
1995-1999, The University of Tennessee. Ph.D., Microbiology
1999-2001, Postdoc, National Institute of Agrobiological Sciences, Tsukuba, Japan.
2002-2006, NIH Postdoc, Department of Plant & Microbial Biology, UC Berkeley.

Research Interests

My research career, from Ph.D. to faculty at MSU, has been guided by the underlying question of how plants interact with microbes. As biochemists, we are interested in the enzymatic activity of both plant and pathogen proteins associated with immune signaling activation and pathogen virulence. Through genetics-based approaches, we use in planta methods to discover and define plant processes required for immunity. Finally, using high-resolution microscopy-based approaches, we quantitatively define changes in the temporal and spatial organization of the plant cell during pathogen infection.

Selected Publications (past 7 years; for a complete list, click here)

Chiang, Y-H., Zhang, M., Toruño, T.Y., Lee, D., Yadeta, K.A., Lemos, M., Lu, Y-J., Lal, N.K., Ma, S., Liu, J., Day, B., Dinesh-Kumar, S.P., Dehesh, K., and Coaker, G. (2018). The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants. Cell Host Microbe. 24: 379-391.

Baetsen-Young, A.M., Vasher, M., Matta, L.L., Colgan, P., Alocilja, E.C., and Day, B. 2018. Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles. Biosensor and Bioelectronics. 101: 29-36.

Guo, J., Islam, M.A., Lin, H., Ji, C., Duan, Y., Peng, L., Zeng, Q., Day, B., Kang, Z., and Guo, J. (2018). Genome-wide identification of cyclic nucleotide-gated ion channel gene family in wheat and functional analyses of TaCNGC14 and TaCNGC16. Front. Plant Sci., 22

Li, P. and Day, B. (2019). Battlefield Cytoskeleton: Cytoskeletal regulation and pathogen targeting of plant immunity. Mol Plant-Microbe Interact.

Zhang, L., Paasch, B.C., Chen, J., Day, B., and He, S.Y. 2019. An important role of L-fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. doi:10.1111/nph.15639.

Lu, Y-J., and Day, B. (2017). Quantitative evaluation of plant actin cytoskeletal parameters during immune signaling. Methods in Mol. Biol. 1578: 207-221.

Shimono, M., Higaki, T., Kaku, H., Shibuya, N., Hasezawa, S., and Day, B. (2016). Quantitative evaluation of stomatal cytoskeletal patterns during the activation of immune signaling in Arabidopsis thaliana. PLoS One. 11:e0159291.

Shimono, M*., Lu, Y*., Porter, K., Kvitko, B., Creason, A., Henty-Ridilla, J., He, S.Y., Chang, J.H., Staiger, C., and Day, B. (2016). The Pseudomonas syringae type III effector HopG1 induces actin filament remodeling in Arabidopsis in association with disease symptom development. Plant Physiol. 171: 2239-2255. (* Equal contribution).

Burkhardt, A. and Day, B. (2015). Transcriptome and small RNAome dynamics during a resistant and susceptible interaction between cucumber and downy mildew. Plant Genome. doi: 10.3835/plantgenome2015.08.0069

Burkhardt, A., Buchanan, A., Cumbie, J.S., Savory, E.A., Chang, J.H.*, and Day, B.* (2015). Alternative splicing in the obligate biotrophic oomycete pathogen, Pseudoperonospora cubensis. Mol. Plant-Microbe Interact. 28: 298-309. *co-corresponding authors.

Corrion, A., and Day, B. (2015). Pathogen resistance signaling in plants. In: eLS. John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/9780470015902.a0020119.pub2. pp. 1-14.

Li, J., Henty-Ridilla, J.L., Staiger, B.H., Day, B., and Staiger, C.J. (2015). Capping protein integrates      multiple MAMP signaling pathways to modulate actin dynamics during plant innate immunity. Nature Comm.  28: 7206. doi: 10.1038/ncomms8206.

Henty-Ridilla, J.L., Li, J., Day, B., and Staiger, C.J. (2014). ADF4 regulates actin dynamics during innate    immune signaling. Plant Cell. 26: 340-352

Henty, J.L., Shimono, M., Li, J., Chang, J.H., Day, B.*, and Staiger, C.J.* (2013). The plant actin cytoskeleton is a novel component of innate immunity. PLoS Path. 9: e1003290. doi:10.1371/journal.ppat.1003290. *co-corresponding authors.

Porter, K., Shimono, M., Tian, M., and Day, B. (2012). Arabidopsis Actin-depolymerizing Factor-4 links pathogen perception, defense activation and transcription to cytoskeletal dynamics. PLoS Pathogens, 8(11): e1003006. doi:10.1371/journal.ppat.1003006 

Varbanova, M., Porter, K., Lu, F., Ralph, J., Hammerschmidt, R., Jones, A.D., and Day, B. (2011). Molecular and biochemical basis for stress induced accumulation of free and bound p-coumaraldehyde in Cucumis sativus. Plant Physiol. 157: 1056-1066.

Day, B., Henty, J., Porter, K., and Staiger, C. (2011). The pathogen-actin connection: A platform for defense signaling in plants. Ann. Rev. Phytopathol. 49: 483-506.

Knepper, C., Savory, E., and Day, B. (2011). Arabidopsis NDR1 is an integrin-like protein with a role in nutrient stasis and plasma membrane-cell wall adhesion. Plant Physiol. 156: 286-300.

Tian, M., Win, J., Savory, E., Burkhardt, A., Held, M., Brandizzi, F., and Day, B. (2011). 454 genome sequencing of Pseudoperonospora cubensis reveals effector proteins with a putative QXLR translocation motif. Mol. Plant-Microbe Interact. 24: 543-553.

Savory, E.A., Granke, L.L., Quesada-Ocampo, L.M., Varbanova, M., Hausbeck, M.K., and Day, B. (2011). The cucurbit downy mildew pathogen Pseudoperonospora cubensis. Mol. Plant Pathol. 12: 217-226.